ISOLASI, PURIFIKASI DAN UJI PATOGENISITAS ISOLAT Bacillus thuringiensis Berliner WILAYAH DAERAH ISTIMEWA YOGYAKARTA TERHADAP LARVA NYAMUK Aedes aegypti Linn.

Jati, Wibowo Nugroho and Murwani, Indah and Zahida, Felicia ISOLASI, PURIFIKASI DAN UJI PATOGENISITAS ISOLAT Bacillus thuringiensis Berliner WILAYAH DAERAH ISTIMEWA YOGYAKARTA TERHADAP LARVA NYAMUK Aedes aegypti Linn. [Research]

Text (Penelitian teknobiologi) BL40205.pdf Restricted to Registered users only Download (7MB)

Abstract

Every year dengue disease which is caused by bites of Aedesaegypti mosquito mostly end in mortality. Spread prevention of this disease need much comprehensive handling. One way to eradicate larvae of mosquito A.aegypti is using its natural enemy Bacillus thuringiensis. Research has been done in Yogyakarta Special Province area which is including Yogyakarta City, Sleman, Gunungkidul, Bantul, and Kulonprogo districts. Research has been done in Technobiology Laboratory, Faculty of Technobiology, University of Atma Jaya Yogyakarta. Isolation of B.thuringiensis was using Ohba and Aizawa method. There are two phase of research. First phase was selective isolation, identification, iv gram staining, and screening test of killing power of strain B.thuringiensis to Ae. aegypti’s larvae, pathogenicity test and probit analysis. The second phase was selection of B.thuringiensis which its virulence bigger or similar with 80%, isolate multiplication of virulent B.thuringiensis, pathogenicity test of B thuringiensis which is most virulent and laboratory scale bio insecticide production. Results of first phase showed that 19 of 74 isolates of B thuringiensis have a virulence of 80%. Pathogenicity test LC 50/ 24 hrs., showed effective concentration of 2.04 x 105. Results of the pathogenicity test of the second phase showed that one of 19 isolates was strongly virulent. The most pathogenic isolate was 75P. Isolate 75P can be used as raw material for natural bioinsecticide.

Actions (login required)

View Item